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T2 (174 x CEM.T2) - CRL-1992 | ATCC
T2 (174 x CEM.T2) cellls were derived from a variant of the T1 cell line (ATCC CRL-1991) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR). The cell line can be used as a transfection host and for studying antigen processing and T cell recognition of class I MHC antigens.
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Культура клеток — Википедия
Культивирование клеток представляет собой процесс, посредством которого in vitro отдельные клетки (или единственная клетка) прокариот и эукариот выращиваются в контролируемых условиях. На практике термин «культура ...
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Humoral and cellular immune memory to four COVID-19 vaccines: Cell
Longitudinal analysis of T cell, B cell, and antibody response to four different COVID-19 vaccines in humans, representing three different vaccine technologies, identifies different quantities and quality of CD4+ T cell, CD8+ T cell, and antibody response elicited by mRNA-1273, BNT162b2, Ad26.COV2.S, and NVX-CoV2373. Antibody levels decline, while memory T cells and B cells …
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Cell culture - Wikipedia
Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies ...
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Inactivation of the Hippo tumor suppressor pathway promotes
Jun 29, 2022 · Primary adult epidermal melanocytes were purchased from ATCC (PCS-200-013) and maintained in Dermal Cell Basal Medium (ATCC PCS-200-030) supplemented with an Adult Melanocyte Growth Kit (ATCC PCS ...
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Humoral and cellular immune memory to four COVID-19 vaccines
Jul 07, 2022 · The dotted line indicates the limit of quantification (LOQ). ... (C-D) or T2 (E-F) with memory B cell and antibody data from the late timepoint. ... Experimental models: Cell lines; VERO cells: ATCC: ATCC CCL-81: HEK293T cells: ATCC: ATCC CRL-3216: Software and algorithms; Flowjo 10.8.1:
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Caloric Restriction Reprograms the Single-Cell Transcriptional
Cell-cell communication was predicted based on the scRNA-seq data by using CellPhoneDB software (version 1.1.0) (www.cellphonedb.org) (Vento-Tormo et al., 2018). Only receptors and ligands expressed in at least 10% of cells of a given type were further analyzed, whereas the interaction was considered nonexistent if either the ligand or the ...
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A stromal Integrated Stress Response activates perivascular cancer
Jun 02, 2022 · Rosa26::CreER T2:Atf4 WT/WT and Rosa26:: ... (American Type Culture Collection (ATCC), ... B. Stanger for providing the pancreatic cancer cell line; and staff at the University of Pennsylvania ...
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JCI - RBMS1 regulates lung cancer ferroptosis through translational
Oct 05, 2021 · Viruses were harvested 72 hours after transfection. A549 and NCI-H1299 cells were infected for 24 hours followed by puromycin (5 μg/mL) selection. For CRISPR knockout cell construction, sgRNA was introduced into the lentiCRISPRv2 vector and packaged in lentivirus, and infected cells were screened with puromycin to obtain the stable cell line.
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Sunitinib Dose Escalation Overcomes Transient Resistance in Clear Cell …
Feb 09, 2015 · Abstract. Sunitinib is considered a first-line therapeutic option for patients with advanced clear cell renal cell carcinoma (ccRCC). Despite sunitinib's clinical efficacy, patients eventually develop drug resistance and disease progression. Herein, we tested the hypothesis whether initial sunitinib resistance may be transient and could be overcome by dose increase. …
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