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Proteinase K - Wikipedia
https://en.wikipedia.org/wiki/Proteinase_K
WEBProteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
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An Introduction to Proteinase K and Its Applications - MilliporeSigma
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/dna-and-rna-purification/introduction-to-proteinase-k-and-its-applications
WEBProteinase K is a serine protease that breaks down proteins by hydrolyzing peptide bonds. It is a broad-spectrum protease that can digest a wide variety of proteins, including those that are resistant to other proteases. Proteinase K is commonly used in molecular biology and biochemistry applications to digest structural proteins and enzymes.
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Proteinase K, Molecular Biology Grade | NEB
https://www.neb.com/en/products/p8107-proteinase-k-molecular-biology-grade
WEBProteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates. Highly characterized for more consistent performance. Optimal activity and stability for up to …
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Guide on How to Use Proteinase K in Different Procedures
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/dna-and-rna-purification/guide-to-use-proteinase-k-procedures
WEBProteinase K (Pro K, ProK) is a commonly used protease enzyme in molecular biology, often used for DNA or RNA extraction or for protein digestion. To prepare a stock solution of Proteinase K, follow these steps: 1. Weigh out the desired amount of Proteinase K powder using a balance.
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Proteinase K - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/proteinase-k
WEBProteinase K. Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C.
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Proteinase K : Definition, Activity and Uses - Microbiologie clinique
https://microbiologie-clinique.com/laboratory-proteinase-k.html
WEBProteinase K is a broad-spectrum, nonspecific, proteolytic enzyme widely used in molecular biology to inactivate protein contaminants in prepared samples. This endopeptidase remains active over a wide pH range (optimal between 6.5 and 9.5) and relatively high temperatures (optimal 50-65°C).
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Proteinase K for diverse molecular biology applications
https://www.selectscience.net/editorial-articles/proteinase-k-provides-versatility-in-molecular-biology/?artID=60599
WEBMar 6, 2024 · Proteinase K is a serine protease that is renowned for its proficiency in breaking down proteins through the hydrolysis of peptide bonds. Its versatile nature enables the digestion of a wide spectrum of proteins, even those resistant to alternative proteases.
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Direct Clinical Evidence Recommending the Use of Proteinase K …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530184/
WEBSep 18, 2020 · Direct Clinical Evidence Recommending the Use of Proteinase K or Dithiothreitol to Pretreat Sputum for Detection of SARS-CoV-2 - PMC. Journal List. Front Med (Lausanne) PMC7530184. As a library, NLM provides access to scientific literature.
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Proteinase K (EC 3.4.21.14) | SpringerLink
https://link.springer.com/protocol/10.1385/0-89603-234-5:305
WEBMar 4, 2021 · Abstract. Proteinase K is a serine protease and the main proteolytic enzyme produced by the fungus Tritirachium album Limber ( 1 ). The enzyme has abroad specificity, cleaving peptide bonds C-terminal to a number of amino acids.
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Extracellular protease digestion to evaluate membrane protein …
https://www.nature.com/articles/nprot.2015.131
WEBNov 19, 2015 · 4 Altmetric. Metrics. Membrane proteins have crucial roles in signaling and as anchors for cell surface display. Proper secretion of a membrane protein can be evaluated by its susceptibility to...
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