Keyword Analysis & Research: mc3t3 e1

wikipedia.org

https://en.wikipedia.org/wiki/MC3T3

MC3T3 is an osteoblast precursor cell line derived from Mus musculus (mouse) calvaria. A number of derivatives of this strain have been isolated to select for varying degrees of osteogenic potential, and have been widely used as model systems in bone biology. A standard textbook calls its MC3T3-E1 sub-line "one of the most convenient and physiologically relevant systems for study of transcriptional control in calvarial osteoblasts." This is a spontaneously trans…

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atcc.org

https://www.atcc.org/products/crl-2593

The MC3T3-E1 Subclone 4 ( ATCC CRL-2593) and the MC3T3 Subclone 14 ( ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix (ECM) after 10 days. Ref

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atcc.org

https://www.atcc.org/products/crl-2594

The MC3T3-E1 Subclone 4 ( ATCC CRL-2593) and the MC3T3 Subclone 14 ( ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix (ECM) after 10 days.

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nih.gov

https://pubmed.ncbi.nlm.nih.gov/34299372/

Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions ... Author: Makoto Izumiya, Miyu Haniu, Katsuya Ueda, Haruka Ishida, Chuang Ma, Hirokazu Ideta, Atsushi Sobajima... Publish Year: 2021

Author: Makoto Izumiya, Miyu Haniu, Katsuya Ueda, Haruka Ishida, Chuang Ma, Hirokazu Ideta, Atsushi Sobajima...

Publish Year: 2021

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sciencedirect.com

https://www.sciencedirect.com/topics/medicine-and-dentistry/mc3t3-e1-cell-line

Both PP and Y4 receptor are found in the transformed murine osteoblastic cell line MC3T3-E1, with PP treatment stimulating differentiation of these cells, suggesting a role for PP in osteoblast differentiation [45]. This is supported by findings from Y4 receptor knockout mice, in which osteoblast number is decreased [46].

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creative-bioarray.com

https://www.creative-bioarray.com/MC3T3-E1-CSC-C3037-item-1623.htm

MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA. Species Mouse Source Calvaria Recommended Medium SuperCult® AlphaMEM + 2mM Glutamine + 10% h.i. FBS Culture Properties adherent Morphology Fibroblast-like Karyotype Murine hypotetraploid karyotype with 11.5% polyploidy - 72 (68-80)<4n> - centric fusion absent

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nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691241/

Jul 03, 2019 · The treated MC3T3-E1 cells were plated in 6-cm culture dishes and maintained in the presence of 5% CO 2 at 37°C. After cell confluence reached >90%, a cell-free line was created by a 200 µl sterile pipette tip.

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nih.gov

https://pubmed.ncbi.nlm.nih.gov/18573985/

To verify this hypothesis, we cultured osteoblast-like MC3T3-E1 cells on these CaP coatings. It was found that MC3T3-E1 cells cultured on the electrodeposited CaP/chitosan coatings had cell proliferation rates higher than those on the electrodeposited CaP coatings.

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nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042760/

Sep 06, 2016 · MC3T3-E1 cells were cultured in the osteogenic differentiation medium for 14 days, following by culture in DMEM supplemented with varying concentrations of liraglutide (catalog no. HY-P0014; MedChem Express, Shanghai, China) for a further 14 days.

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