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NCTC clone 929 [L cell, L-929, derivative of Strain L] | ATCC
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Temperature. 37°C. Atmosphere. 95% Air, 5% CO 2. Handling procedure ...
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Growth and Maintenance of Vero Cell Lines - PMC
At its 93 rd passage, the cell line was brought to the National Institute of Allergy and Infectious Diseases at the National Institutes of Health in the United States, and was provided to the American Type Culture Collection (ATCC) in 1966. By the end of the 1960s, Vero cell lines were being used across the globe, primarily in virology ...
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HEK293细胞ATCC CRL-1573细胞HEK293人胚胎肾，腺病毒5型转 …
Exome-based proteogenomics of HEK-293 human cell line: coding genomic variants identified at the level of shotgun proteome. ... NG108-15细胞ATCC HB-12317; NCTC clone 929细胞L929细胞ATCC CCL-1; RAW 264.7细胞ATCC TIB-71; Hs 578Bst细胞ATCC HTB-125; OA3.Ts细 …
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Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, …
Jan 01, 2021 · The human monocytic cell line THP-1 (ATCC, TIB-202) was cultured in RPMI media (Corning, 10-040-CV) supplemented with 10% FBS and 1% penicillin and streptomycin. The primary umbilical vein endothelial cells from normal human (HUVEC) (ATCC, PCS-100-013) were cultured in vascular cell basal medium (ATCC, PCS-100-030) containing cell growth ...
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The gut microbiota prime systemic antiviral immunity via the cGAS …
May 10, 2022 · L929 cells, murine fibroblasts, were maintained in DMEM with 10% HI-FBS and split in a ratio of 1:2 to 1:8 when cell confluency of 95% was reached. To produce L929-conditional medium, 1×10 6 L929 cells were cultured in 50 ml DMEM with 10% HI-FBS in 175 cm 3 flasks for 1 week, then conditional medium was harvested and sterile-filtered (0.2 μm ...
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Alloyed nanostructures integrated metal-phenolic ... - ScienceDirect
Cytotoxicity test of the membranes were carried with CCK-8 assay with L929 cells. L929 cells were grown in complete medium containing DMEM. 100 μL of the cells was seeded on a 24-well tissue culture polystyrene plate (control) or membranes at a density of 0.7 × 10 4 cells/cm 2. After 1 h, another 400 μL of the culture medium was added.
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Frontiers | Plasma Membrane Localization of CD36 Requires …
SW13 cells were purchased from ATCC (ATCC, United States) and incubated following their protocol. ... We differentiated murine bone marrow myeloid stem cells into BMDMs by culturing in DMEM supplemented with 10% L929 supernatant and 10% fetal bovine serum (FBS) for 7 days. Harversen et al. used high-glucose DMEM supplemented with 10% whole ...
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Novel Graphene Oxide/Quercetin and Graphene Oxide/Juglone
Jun 06, 2022 · In this paper, novel drug delivery systems (DDS) were designed based on graphene oxide (GO) as nanocarrier, loaded with two natural substances (quercetin (Qu) and juglone (Ju)) at different concentrations. The chemical structure and morphology of the synthesized GO-based materials were characterized by Fourier transform infrared …
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Injectable antibacterial conductive nanocomposite cryogels
Jul 17, 2018 · The nanocomposite cryogel without X-ray detectable line was used as a control. ... The cytotoxicity of the cryogels on L929 (ATCC CCL-1, the L929 cell was purchased from the Shanghai Cell Bank of ...
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Dysregulation of macrophage PEPD in obesity determines adipose …
Apr 25, 2022 · To make L929-conditioned cell medium, L929 cells (CCL-1, American Type Culture Collection (ATCC)) were seeded in DMEM supplemented with 10% heat-inactivated FBS, 100 U ml −1 penicillin ...
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