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Moreover, in Granta-519 cells that was characterized by overexpression of cyclin D1. the degrader BSJ-02-162 or BSJ-03-204 could result in, marked degradation of CDK4/6and induced G1 cell cycle ...
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SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen ... - Nature
Aug 05, 2020 · In brief, heat-inactivated serum was mixed with pseudoviruses, incubated, and then added to Huh7.5 cells or ACE-2-expressing 293T cells for MERS-CoV and SARS-CoV-2 respectively.
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Genome-wide bidirectional CRISPR screens identify mucins as host
Jul 25, 2022 · The virus was rescued and passaged in Huh7.5.1 cells until widespread GFP fluorescence and CPEs were observed. Virus was propagated on Vero E6 cells and titrated on Vero E6 cells overexpressing ...
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Rottlerin plays an antiviral role at early and late steps of Zika virus
Aug 05, 2022 · Next, we tested the effect of rottlerin on ZIKV replication in another two human cell lines, SNB19 and Huh7.5 cells. The data showed that the IC 50 of rottlerin against ZIKV in SNB19 cells and Huh7.5 cells were 0.19 μmol/L and 0.70 μmol/L, respectively ( Fig. 1 C and D), demonstrating that the antiviral effect of rottlerin was not cell-specific.
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HIF1A hypoxia inducible factor 1 subunit alpha [ (human)]
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and …
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The human disease gene LYSET is essential for lysosomal enzyme
Sep 08, 2022 · A.R. performed SARS-CoV-2-mNeon infections and flow cytometry supervised by C.A.B. R.B. provided A549-ACE2 cells. A.P. provided Huh7.5.1-ACE2/TMPRSS2 cells. J.E.C. and T.B. designed and supervised the research, interpreted the data, and prepared the manuscript. All authors provided comments for the manuscript and agreed on the final version.
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7422 - Gene ResultVEGFA vascular endothelial growth factor A
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel …
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RNeasy Kits - Qiagen
Huh7: 5 x 10 5 – – – 1 x 10 5: 7.5mg ... Cells can be grown and directly lysed in 96-well cell-culture dishes. After transfer of the lysates to the wells of the RNeasy 96 plate, RNA binds to the silica membrane in each well, and contaminants are washed away. Pure RNA is then eluted in RNase-free water into individual collection tubes ...
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Identification of SARS-CoV-2 inhibitors targeting Mpro and
Feb 25, 2022 · Huh7.5 cells were treated with the inhibitors as shown, and reduction in virus titer at 24 h.p.i. was determined by plaque assays. Data are shown as mean ± SEM. p Values ...
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GEO Accession viewer - National Center for Biotechnology Information
Feb 21, 2020 · Transcriptomic landscape of developing Presomitic Mesoderm (PSM) from Tailbud to somite in E9.5 mouse embryo and in in vitro differentiated Paraxial mesoderm derived from mouse embryonic stem cells (mESCs). GSE44039: TET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells: GSE44532
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