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Inhibition of ASGR1 decreases lipid levels by promoting ... - Nature
Aug 03, 2022 · Huh7 cells were transfected with 2 μg pX330 along with pLKO.1, which provided puromycin resistance. The cells were screened with 5 μg ml −1 puromycin for 48 h. The mixture was then seeded into ...
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Deubiquitinase-targeting chimeras for targeted protein ... - Nature
Feb 24, 2022 · Hep3B2.1-7 (HEP3B) cells were purchased from ATCC (HB-8064) and cultured in EMEM (ATCC) containing 10% (vol/vol) FBS and maintained at 37 °C with 5% CO 2. Gel-based activity-based protein profiling
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Programmable deletion, replacement, integration and inversion …
Dec 09, 2021 · Huh7 cells (a gift from Erik Sontheimer’s group, originated from ATCC) were cultured and passaged in DMEM plus GlutaMAX (Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Gibco ...
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GCN2 inhibition sensitizes arginine-deprived ... - Cell Metabolism
Jul 14, 2022 · Based on previous reports, SNU-449 and Huh7 express relatively more p53 protein than HepG2 and Hep3B cells, and p53 is essentially undetectable in SNU-398 (Sayan et al., 2001. Sayan A.E. Sayan B.S. Findikli N. Ozturk M. Acquired expression of transcriptionally active p73 in hepatocellular carcinoma cells. Oncogene. 2001; 20: 5111-5117.
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Aquaporins: Important players in the cardiovascular pathophysiology
Sep 01, 2022 · 1. Introduction. A 28KD hydrophobic transmembrane protein CHIP28 existing on the erythrocyte membrane was uncovered by Agre in 1988, which was subsequently confirmed to be a specific water-transport channel protein on the membrane and renamed aquaporin 1 , .Up to now, 13 kinds of AQPs have been identified in mammalian tissues and are classified into three …
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Mammalian lipid droplets are innate immune hubs integrating cell
Oct 16, 2020 · The bacterial strains used were: E. coli (ATCC 25922), MRSA (strain 162057-900), P. aeruginosa (ATCC 27853), and L. monocytogenes (strain 10403S). HEK293 or HEK293-TLR4 + cells were seeded at 4 × 10 5 cells/plate in 6-well plates and transfected the next day. Six hours after transfection, culture media was replaced for antibiotic-free cell ...
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Feeding induces cholesterol biosynthesis via the mTORC1
Nov 11, 2020 · Cholesterol is an essential lipid and its synthesis is nutritionally and energetically costly1,2. In mammals, cholesterol biosynthesis increases after feeding and is inhibited under fasting ...
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Rational development of a combined mRNA vaccine against …
Jul 26, 2022 · HEK293T (ATCC) cells were seeded ... One hour later, Huh7 cells were added to 96-well plates at 3.5 × 10 4 cells per well and incubated at 37 °C for 24 h. The supernatants were removed, and ...
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LYTACs that engage the asialoglycoprotein receptor for ... - Nature
Mar 25, 2021 · HEP3B cells (ATCC), HUH7 cells (JCRB) and HeLa–GFP cells (MyBioSource) were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin. HEPG2 cells (ATCC) were cultured in DMEM ...
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