Keyword Analysis & Research: hek293t cells pfizer


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What are HEK293 cells?

HEK293 cells are Human Embryonic Kidney cells, originally isolated and grown by Dutch biologist Alex Van der Eb in the early 1970s. They were transfected with sheared adenovirus 5 (Ad5) DNA by Frank Graham, a postdoc in Van der Eb’s lab. It was his 293rd experiment, which is why they got the tag HEK293. (1)

What is the difference between hek293-e and Hek 293-6e?

HEK293-E was established by the expression of Epstein-Barr nuclear antigen 1 (EBNA-1), which allows for episomal replication of plasmids with oriP. Similarly, the HEK293-6E cell line was established by the expression of a truncated EBNA-1, lacking Gly-Gly-Ala domain.

Are HEK293T cells and adenoviral cells the same thing?

Incorporating the adenoviral genes into the HEK cell genome resulted in the cells becoming very efficient at producing high amounts of recombinant proteins from plasmid vectors carrying the CMV promoter region. So, are HEK293T cells the same thing? Not exactly.

How did HEK cells become so efficient at producing recombinant proteins?

Incorporating the adenoviral genes into the HEK cell genome resulted in the cells becoming very efficient at producing high amounts of recombinant proteins from plasmid vectors carrying the CMV promoter region. So, are HEK293T cells the same thing?


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