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Frequently Asked Questions
The HEK293 cell line is a permanent line established from primary embryonic human kidney, which was transformed with sheared human adenovirus type 5 DNA. The adenoviral genes expressed in this cell line allow the cells to produce very high levels of recombinant proteins.Do cell culture environments affect the morphological and functional properties of HEK293T cells?
Human embryonic kidney 293T (HEK293T) cells are used in various biological experiments and researches. In this study, we investigated the effect of cell culture environments on morphological and functional properties of HEK293T cells. We used several kinds of dishes made of polystyrene or glass for …Do you offer HEK293 cell lines for high-density suspension culture?
We offer several variants of the HEK293 cell line, including those adapted for high-density suspension culture in serum-free media. For more information, visit the mammalian cell culture pages on our website.What are the incubation conditions for HEK293 cells?
The needs of HEK293 cells are pretty simple. Non-negotiables include a humidified incubator kept at 37°C with 5% CO 2 and a diet of high-glucose media such as Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with Fetal Bovine Serum (FBS).