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Search-and-replace genome editing without double-strand breaks ... - Nature
Oct 21, 2019 · HEK293T cells were seeded in 48-well plates and transfected at approximately 70% confluency with 750 ng editor plasmid (PE2, PE2(R110S/K103L), Cas9(H840A) nickase, or dCas9), 250 ng HEK3-targeting ...
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FcγR-mediated SARS-CoV-2 infection of monocytes activates
Apr 06, 2022 · Infected HEK293T cells were used as a positive control ... By contrast, plaques were easily detected in culture supernatants from infected Vero cells collected at 48 h after infection. Thus ...
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Package of Lenti-shRNA virus using HEK293T cells - MD …
1. Before seeding cells for virus package, make sure HEK293T cells are in rapid replication state by growth cells to approximately 75%~80% confluence in a 10 cm cell culture dish and passaging at 1:2 ratio for at least 2 consecutive days; 2. On day 0, Seed HEK293T cells at 8.5~9´106 cells in growth medium without antibiotics for a 10- cm
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293T - CRL-3216 | ATCC
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended.
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KDM5B promotes immune evasion by recruiting SETDB1 to silence ... - Nature
Oct 20, 2021 · KDM5B recruits SETDB1 to repress endogenous retroelements such as MMVL30, suppressing anti-tumour immunity, and the depletion of KDM5B induces a robust adaptive immune response and enhances the ...
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Establishment of a HEK293T cell line able to site-specifically
HEK293T cells purchased from cell bank of Typical culture preservation commission, Chinese academy of sciences, were cultured in 24-well plates filled with 90% Dulbecco's modified eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Grand Island, NY, USA).
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HEK 293 cells - Wikipedia
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973.. HEK 293 cells have been widely used in cell biology research for many …
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Post-translational control of beige fat biogenesis by PRDM16
Aug 17, 2022 · Cell culture. The stromal vascular ... HEK293T cells, HEK293 virus packaging cells and C2C12 cells were maintained in DMEM high-glucose medium containing 10% FBS and 1% penicillin–streptomycin.
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FectoVIR®-AAV for AAV virus production in suspension cells
Description High performance: Superior yields for rAAV production in suspension culture system. FectoVIR ®-AAV transfection reagent is a novel chemical-based and animal-free transfection reagent that has been engineered to address the current limits in rAAV viral vector yields and industrial scalability.FectoVIR ®-AAV transfection reagent is the outcome of an extensive …
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Gasdermin E suppresses tumour growth by activating anti-tumour ... - Nature
Mar 11, 2020 · Cell lines and cell culture conditions. HeLa, HEK293T and EMT6 cells were purchased from ATCC. 4T1E and 4T1E–eGFP cells were generated in our laboratory by sorting 4T1 cells for high E-cadherin ...
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