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ExpressCells | Cell Line Gene Editing
Web“The ExpressCells CRISPR-edited cell line contributed greatly to our COVID-19 related work and will eventually lead to a publication. Not only did Express Cells deliver the cells quickly, the CRISPR-edited cell pool exhibited such high level overexpression that a clonal cell line was not required. We appreciate the quality of Express Cells ‘ work and their team’s …
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Search-and-replace genome editing without double-strand breaks ... - Nature
WebOct 21, 2019 · HEK293T cells were seeded in 48-well plates and transfected at approximately 70% confluency with 750 ng editor plasmid (PE2, PE2(R110S/K103L), Cas9(H840A) nickase, or dCas9), 250 ng HEK3-targeting ...
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293T - CRL-3216 | ATCC
WebThe 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Age fetus Comments This cell line is competent to replicate vectors carrying the SV40 region of …
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HEK 293 cells - Wikipedia
WebHuman embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973.. HEK 293 cells have been widely used in cell …
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Package of Lenti-shRNA virus using HEK293T cells - MD …
Web1. Before seeding cells for virus package, make sure HEK293T cells are in rapid replication state by growth cells to approximately 75%~80% confluence in a 10 cm cell culture dish and passaging at 1:2 ratio for at least 2 consecutive days; 2. On day 0, Seed HEK293T cells at 8.5~9´106 cells in growth medium without antibiotics for a 10- cm
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Establishment of a HEK293T cell line able to site-specifically
WebThe infected HEK293T cells expressing EGFP were separated by the LSRFortessa flow cytometer and separately cultivated in 96-well plates filled with 90% DMEM with 10% FBS. The cell culture media were changed every two days. When the cells spread to 80% of the plate, they were transferred to 24-well plates to expand the culture dose and establish ...
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KDM5B promotes immune evasion by recruiting SETDB1 to silence ... - Nature
WebOct 20, 2021 · KDM5B recruits SETDB1 to repress endogenous retroelements such as MMVL30, suppressing anti-tumour immunity, and the depletion of KDM5B induces a robust adaptive immune response and enhances the ...
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Post-translational control of beige fat biogenesis by PRDM16
WebAug 17, 2022 · HEK293T cells, HEK293 virus packaging cells and C2C12 cells were maintained in DMEM high-glucose medium containing 10% FBS and 1% penicillin–streptomycin. Plasmids.
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Genes to Cells - Wiley Online Library
WebSep 18, 2022 · Local anesthetic lidocaine induces growth suppression of HeLa cells by decreasing and changing the cellular localization of the proliferation marker Ki-67. ... First Published: 8 September 2022; Abstract. Local anesthetic lidocaine induced growth suppression of HEK293T and HeLa cells. Lidocaine decreased Ki-67 expression and …
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PROBER identifies proteins associated with programmable …
WebAug 04, 2022 · HEK293T cells (TaKaRa) were maintained in DMEM (Gibco) supplemented with 10% FBS and 1% penicillin–streptomycin at 37 °C with 5% CO 2, and were transfected with Lipofectamine 3000 (Invitrogen).
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