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HEK293 Cell Culture and Transfection | HEK293 Cell Line
HEK293 are rounded cells that grow in suspension in cell culture, although initially they were an adherent cell line. HEK-293 cells should be grown in a complete SFMII growth medium supplemented with 4 mM L-glutamine. Flasks should be incubated at 37°C in 5% CO2 and HEK293 cell doubling time is approximately 34 hours. Subculture Protocol for ...
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HEK293: An Essential Human Cell Line with a Unexpected Origin
Both HEK293 and HEK293T represent adherent cell lines that form a stable connection with coated cell culture dishes. This limits their volume to a two-dimensional space. Therefore, HEK293F cells were selected to be cultured in suspension by adaptation, elevating them to the three-dimensional space and allowing for more efficient growth of high ...
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HEK293 Cells - A Guide to This Incredibly Useful Cell Line
Sep 27, 2021 · Keeping Them Happy. The needs of HEK293 cells are pretty simple. Non-negotiables include a humidified incubator kept at 37°C with 5% CO 2 and a diet of high-glucose media such as Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with Fetal Bovine Serum (FBS). Culturing media can also contain broad-spectrum antibiotics such as Penicillin …
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A proximity-dependent biotinylation map of a human cell | Nature
Jun 02, 2021 · For the generation of stable cell lines, HEK293 Flp-In T-REx cells were transfected using the jetPRIME transfection reagent (Polyplus CA89129-924). Cells were seeded at 250,000 cells per well in a ...
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Structures and gating mechanisms of human bestrophin anion …
Jul 04, 2022 · The full-length (FL) and truncated channels were individually transfected into HEK293 cells and subjected to whole-cell patch clamp recording in the absence and presence of 1 …
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Cell Counting Kit-8 | Dojindo
Cell Line Description Publication 10T1/2 mouse embryonic fibroblast ... HEK293 human embryonic kidney cell J. Hyung, et al., Mol. Cancer Res. 8, 246-253 (2010) ... How stable is CCK-8? CCK-8 is stable over 6 months at room temperature and one year at 0-5 o C with protection from light.
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Evolution from adherent to suspension: systems biology of HEK293 cell …
Nov 04, 2020 · The HEK293 cell line was originally immortalized by the random integration of viral genomic DNA of adenovirus 5 18, which includes the E1A and E1B genes.In this study, overall high mRNA levels of ...
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Cell Line Development | Sartorius
Sartorius’ outstanding cell line technology and its experienced team, with more than 120 successfully developed cell lines, anchors our single-vendor approach for cell line development. We cater to the individual needs of our customers at each stage to ensure a successful research cell bank (RCB), master cell bank (MCB), and working cell bank ...
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Viral Vectors - Lonza
We produce AAV using various processes ranging from HEK293-based transient transfection, Hela cell-based producer cell lines with Ad helper infection to recombinant baculovirus-based methods with insect cell lines. Our proprietary clonal HEK293-based suspension processes for transient transfection are designed to be scalable and robust for your ...
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Stable cell line の樹立（大徳 浩照）
Stable cell line の樹立に当たっては、必ずしも高い遺伝子導入効率は求められないが、染色体に挿入される確率を高めるために環状のプラスミドを制限酵素消化で直鎖状にし、精製したものを用いる。遺伝子導入試薬は、当研究室では主にFuGENE6を用いている。
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