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HEK 293 cells - Wikipedia
WebImmunofluorescent HEK 293 cells. HEK 293 cells are straightforward to grow in culture and to transfect. They have been used as hosts for gene expression. Typically, these experiments involve transfecting in a gene (or combination of genes) of interest, and then analyzing the expressed protein.
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HEK293 Cells: Background, Applications, Protocols, and More
WebHEK293 is a robust, fast-growing, and low-maintenance cell line with a variety of applications, including receptor signaling, cancer research, protein production, and CRISPR gene editing. HEK293 cells are immortalized human embryonic kidney cells and are among the most commonly used cell lines in research.
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293 [HEK-293] - CRL-1573 | ATCC
Web293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and …
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HEK293 Cells - A Guide to This Incredibly Useful Cell Line
WebSep 27, 2021 · HEK293 cells are Human Embryonic Kidney cells, originally isolated and grown by Dutch biologist Alex Van der Eb in the early 1970s. They were transfected with sheared adenovirus 5 (Ad5) DNA by Frank Graham, a postdoc in Van der Eb’s lab. It was his 293rd experiment, which is why they got the tag HEK293. (1)
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Evolution from adherent to suspension: systems biology of HEK293 …
WebNov 4, 2020 · The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the...
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HEK 293 Cells: Background, Advantages, and Applications - BioSpace
WebJun 30, 2021 · HEK 293 cells are hypotriploid human cell lines. They carry 64 chromosomes, three X chromosome copies, and an incorporation of 4 kbp Ad5 to chromosome 19 of the HEK 293 genome indicating cytogenetic instability. HEK 293 Cells Culturing Information and Requirements HEK 293 cells are considered a low-maintenance and robust cell line.
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HEK293 Cells - Morphology | Thermo Fisher Scientific - US
WebThe HEK293 cell line is a permanent line established from primary embryonic human kidney, which was transformed with sheared human adenovirus type 5 DNA. Learn more about the healthy HEK293 cell morphology.
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HEK293 Cell Line as a Platform to Produce Recombinant …
WebDec 13, 2021 · Among the human cell lines, HEK293 is widely utilized due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture. In this review, we discuss the use of HEK293 cells and its subtypes in …
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Affecting HEK293 Cell Growth and Production Performance by Modifying
WebJul 2, 2021 · The HEK293 cell line is of epithelial origin and adherent in nature. It has been adapted to grow in suspension culture in serum-free or chemically defined media and is currently being utilized for recombinant proteins expression in …
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HEK293 in cell biology and cancer research: phenotype, …
WebSep 15, 2015 · 293 cell line (widely known as the Human Embryonic Kidney 293 cells) and its derivatives were the most used cells after HeLa in cell biology studies and after CHO in biotechnology as a vehicle for the production of adenoviral vaccines and recombinant proteins, for analysis of the neuronal synapse fo …
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