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293T/17 SF [HEK 293T/17 SF] - ACS-4500 | ATCC
To download a certificate of analysis for 293T/17 SF [HEK 293T/17 SF] (ACS-4500), enter the lot number exactly as it appears on your product label or packing slip. ... ATCC recommends passaging thawed cells at least twice prior to transfection to ensure optimal viability. Prior to transfection (24 hours), seed cells at a density of 8 x 10 5 ...
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293T/17 [HEK 293T/17] - CRL-11268 | ATCC
Sep 14, 1999 · 293T/17 [HEK 293T/17] is a cell line exhibiting epithelial morphology that was isolated from human embryo kidney tissue. The cell line is a derivative of the 293T (293tsA1609neo) cell line, a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the …
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Primary Renal Proximal Tubule Epithelial Cells; Normal, Human ... - ATCC
The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. ATCC Primary Cell Solutions cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability. ... 293T/17 [HEK 293T/17] CRL-11268. 293T. CRL-3216. HK-2. CRL-2190. Detailed product ...
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293 [HEK-293] - CRL-1573 | ATCC
293 [HEK-293] is a cell line that was isolated from the kidney of a human embryo. It can be used in industrial biotechnology and toxicology research and has applications in efficacy and viruscide testing. ... 293T/17 [HEK 293T/17] CRL-11268. Primary Renal Proximal Tubule Epithelial Cells; Normal, Human (RPTEC) ... Shaw G, et al. Preferential ...
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293T - CRL-3216 | ATCC
293T is an epithelial-like cell that was isolated from the kidney of a patient and can be used in ... ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and ... [HEK 293T/17] CRL-11268. Primary Renal Proximal Tubule Epithelial Cells; Normal, Human ...
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HEK 293 cells - Wikipedia
293T (or HEK 293T) is a derivative ... HEK 293, and especially HEK 293T, cells are commonly used for the production of various retroviral vectors. ... ATCC entry for 293T This page was last edited on 19 May 2022, at 20:10 (UTC). Text is available under the …
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HEK293 Cell Line Origins, Cytogenetics, and Expression
HEK 293 cells are popular for their ease of growth and transfection (HEK293 Transfection Kit), making them a common cell culture in cancer research. In addition, high transfection efficiency of HEK293 cells produces exogenous proteins or viruses for pharmaceutical and biomedical research purposes. HEK-293 cells are useful for many transfection experiments, particularly …
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Cell Culture Media: A Review - Labome
Jan 29, 2022 · HEK 293: Epithelial: Human: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS ... for example, DMEM High Glucose H-21 for Lenti-X 293T cells ... cells , T47D, RENCA and CT26.WT cells . ATCC , Athena Enzyme Systems , Lonza , Mediatech , Nacalai Tesque and US Biological also provided RPMI-1640 media. …
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Rixosomal RNA degradation contributes to silencing of Polycomb …
Mar 30, 2022 · Exon- and transcript-level features consistent with the resulting TSS to TES windows for 21,004 active genes in HEK 293T cells were selected from an hg19 reference GTF (GRCh38.99 from Ensembl).
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Lymph node colonization induces tumor-immune tolerance to …
May 06, 2022 · Lentiviral particles were packaged in HEK-293T cells using the pCMV-VSVG and psPAX2 envelope and packaging plasmids. psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454) (Stewart et al., 2003). Cells were transfected with Lipofectamine 2000 (Thermo 11668019) in …
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