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Fusion Protein Linkers: Property, Design and Functionality
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726540/
WEBOct 10, 2013 · Another Gly and Ser rich flexible linker, GSAGSAAGSGEF, was designed by Waldo et al. to express green fluorescent protein (GFP)-fusion proteins for rapid protein-folding assay . The linker sequence avoided large hydrophobic residues to maintain good solubility in aqueous solutions.
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What is the best linker for a fusion protein? | ResearchGate
https://www.researchgate.net/post/What_is_the_best_linker_for_a_fusion_protein
WEBPopular answers (1) Benoît-Joseph Laventie. University of Basel. There are linkers based on the same principle than the (GGGGS)n which I find superior as they don't have repeats that can lead to...
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CRISPR-mediated knock-in in the mouse embryo using long
https://www.sciencedirect.com/science/article/pii/S1046202320302279
WEBJul 1, 2021 · For imaging experiments a flexible fusion linker is advisable, as it allows for optimal biological activity of the respective proteins. Small and polar residues provide good flexibility and solubility [21]. We routinely combine a GS linker with another Gly and Ser rich flexible linker, GSAGSAAGSGEF [22].
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Three-dimensional context rather than NLS amino acid sequence ... - Nature
https://www.nature.com/articles/s41467-017-01057-7
WEBOct 17, 2017 · In contrast, a slightly longer construct of RCC1 (RCC1-long) that bears a 12 residue linker (GSAGSAAGSGEF) between residues S26 and H27, right at the junction between the β-propeller and NLS ...
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Stepwise requirements for polymerases δ and θ in theta ... - Nature
https://www.nature.com/articles/s41586-023-06729-7
WEBNov 15, 2023 · We generated a Polθ construct substituting the non-conserved central domain (899–1,789) with the linker GSAGSAAGSGEF (Polθ ΔCen) and confirmed expression of Polθ ΔCen in Polθ deficient ...
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Lineage tracing and analog recording in mammalian cells by ... - Nature
https://www.nature.com/articles/s41589-021-00769-8
WEBMar 22, 2021 · The 5×Flag was present on a gBlock (IDT), and the 5×GSA linker (four repeats of the sequence GSAGSAAGSGEF and a final repeat with the sequence GSAGSAAGASGEGRP 45) was ordered on a minigene...
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Tuning protein expression using synonymous codon libraries targeted …
https://academic.oup.com/peds/article/24/1-2/123/1471394
WEBNov 3, 2010 · During studies with a previously described recombinant protein folding reporter (Waldo et al., 1999) containing a protein of interest connected via a flexible synthetic 12 amino acid linker sequence (GSAGSAAGSGEF) to the N-terminus of the GFP, we discovered that a plasmid encoding the synthetic linker-GFP fusion (p380-c12) (Fig. 1a) directed by ...
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In vivo capsid engineering of bacteriophages for oriented …
https://pubs.acs.org/doi/suppl/10.1021/acsabm.2c00428/suppl_file/mt2c00428_si_001.pdf
WEBa flexible linker, L2 d1, and a double-stop codon (TAATAA) The flexible linker (GSAGSAAGSGEF) was added between Gp10A and L2d1 to address molecular flexibility issues (ATG— Gp10AnoTAA__flexible linker__L2D1(AAs 1-60)— TAATAA) gp10A-L-L2d1 T7L-L2d1 Produces NLuc-CBM during infection Gp10A …
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Fusion and secretory expression of an exo-inulinase and a d …
https://pubmed.ncbi.nlm.nih.gov/32700446/
WEBJan 30, 2021 · The best linker ligating the two enzymes was a flexible peptide containing 12 residues (GSAGSAAGSGEF). At 55 °C and pH 8.0, and as with the addition of 1 mmol L -1 Mn 2+ , the CSCA-linkerE-DAE fusion enzyme obtained through high cell-density cultivation displayed a maximal exo-inulinase activity of 21.8 U mg -1 and resulted in a yield of 6.3 g ...
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Liver-directed gene therapy corrects neurologic disease in a …
https://www.cell.com/cms/10.1016/j.omtm.2022.04.010/attachment/826150bc-3596-488e-8b57-2ff8ef109be8/mmc1.pdf
WEBGSA represents GSAGSAAGSGEF linker and SIV represents. SIVAQLSRPDPA linker. The sequences of linkers are shown in Table S1. Figure S2. IDUA enzyme activity of AAV8 transduced Huh7 cells. Huh7 cells were transduced. with AAV8 vectors for three days. The cell culture supernatant was collected for IDUA enzyme. activity analysis.
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