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Fite's Acid Fast Stain - Leprosy - University of Utah
https://webpath.med.utah.edu/HISTHTML/MANUALS/FITES.PDF
Web ResultFITE'S ACID FAST STAIN - LEPROSY CONTROL: Leprosy positive tissue. PROCEDURE: 1. Deparaffinize in xylene/peanut oil mixture, 2 changes, 10 minutes each. 2. Drain slides, blot off excess oil. 3. Rinse in distilled water until slide clears. 4. Carbol-fuchsin, 30 minutes, room temperature. 5. Wash in tap water. 6.
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Pathology Outlines - Acid fast / Auramine-rhodamine
https://www.pathologyoutlines.com/topic/stainsacidfast.html
Web ResultSep 5, 2023 · Fite: to detect M. leprae (leprosy) and Rhodococcus ( Diagn Cytopathol 2001;24:244 ); combines peanut / vegetable oil with xylene to minimize exposure of bacteria cell wall to organic solvents and protect precarious acid-fastness of organism ( #1, #2 ) Ellis and Zabrowarny: protocol excludes phenol; procedure …
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Special Stains in Histology - University of Utah
https://webpath.med.utah.edu/HISTHTML/STAINS/STAINS.html
Web ResultA modification of this stain is known as the Fite stain and has a weaker acid for supposedly more delicate M. leprae bacilli. However, much of the lipid in mycobacteria is removed by tissue processing, so this stain can, at times, be very frustrating and cause you to search extensively for organsisms you are sure are in …
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Wade-Fite Staining Technique For Mycobacterium leprae
https://laboratoryinfo.com/wade-fite-staining-technique-for-mycobacterium-leprae/
Web ResultMar 7, 2022 · The Wade-Fite staining technique is a less common special staining useful in Histopathology. It is the modification of Ziehl-Neelsen Staining Method to demonstrate Mycobacterium leprae in tissue sections, which is much less acid and alcohol fast than the tubercle bacilli.
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Pathogen Detection | International Textbook of Leprosy
https://internationaltextbookofleprosy.org/chapter/pathogen-detection
Web ResultFite-Faraco staining is the preferred method for staining M. leprae. Bacterial load (acid-fast bacilli counts) is associated with clinical forms of the disease. Microscopy in general provides low sensitivity in paucibacillary cases and the differentiation of Mycobacterium leprae from other Mycobacterium species is impossible.
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Fite Stain Kit (Microorganism Stain) (ab150668) | Abcam
https://www.abcam.com/products/assay-kits/fite-stain-kit-microorganism-stain-ab150668.html
Web ResultProduct name. Fite Stain Kit (Microorganism Stain) See all Microorganisms kits. Product overview. Fite Stain Kit ab150668 is intended for use in the histological visualization of Mycobacterium Lepra bacillus and Nocardia. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections. Other products for staining …
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Acid-Fast - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/acid-fast
Web ResultAcid fast (Ziehl-Neelsen carbolfuchsin, Fite): Viable microorganisms (M. leprae) stain positively as parallel bacilli or globular masses. Nonviable organisms are broken, beaded, or stain negatively.
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General Principles in the Diagnosis of Infection - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151840/
Web ResultApr 28, 2010 · The modified stain for acid-fast bacteria (Fite-Faraco or Putt's) detects mycobacterial antigens that are sensitive to strong acid, a step in the decolorization of the ZN stain. For this reason, it can be used to screen for all mycobacteria and may be required to detect certain atypical mycobacteria, such as Mycobacterium …
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Acid Fast Bacteria and Acid Fast Staining | Leica Biosystems
https://www.leicabiosystems.com/knowledge-pathway/acid-fast-bacteria-and-acid-fast-staining/
Web ResultThe Fite stain is used to diagnose leprosy and other bacterial infections such as Nocardiosis. This staining method was worked on by others and was also known as the Fite-Faraco method and the Wade-Fite method. Using peanut oil mixed with xylene in the deparaffinization, the oil coats the cell walls and protects the acid …
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Traditional stains and modern techniques for demonstrating
https://ncbi.nlm.nih.gov/pmc/articles/PMC7150110/
Web ResultNov 22, 2018 · The modified Fite procedure is necessary to demonstrate Mycobacterium leprae due to the organism’s fragile, fatty capsule. Acid-fast bacilli including M. leprae bright red
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