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CRISPR/dCas9-Based Systems: Mechanisms and Applications in …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8540305/
WEBSep 29, 2021 · CRISPR/dCas9 is an innovative and emerging technology for functional genomics. It could be used to regulate the transcription of targeted genes without altering their sequence. The CRISPR/dCas9 has now been used to enhance the resistance or tolerance of plants to biotic and abiotic stress and track the chromatin dynamics in live …
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dCas9-based gene editing for cleavage-free genomic knock-in of …
https://www.nature.com/articles/s41556-021-00836-1
WEBFeb 10, 2022 · This cleavage-free gene editor, dCas9–SSAP, promotes the knock-in of long sequences in mammalian cells. The dCas9–SSAP editor has low on-target errors and minimal off-target effects,...
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dCas9: A Versatile Tool for Epigenome Editing - PubMed
https://pubmed.ncbi.nlm.nih.gov/28879853/
WEBHere, we review the use of dCas9 as a novel and versatile tool for fundamental studies on epigenetic landscapes, chromatin structure and transcription regulation, and the potential of this approach in basic research in these fields.
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Elongation roadblocks mediated by dCas9 across human genes …
https://www.nature.com/articles/s41594-023-01090-9
WEBOct 2, 2023 · Abstract. Non-cleaving Cas9 (dCas9) is widely employed to manipulate specific gene loci, often with scant regard for unintended transcriptional effects. We demonstrate here that dCas9 mediates...
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CRISPR/dCas9 Tools: Epigenetic Mechanism and Application in …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573330/
WEBOct 3, 2023 · The dCas9 p300-core (WT) fusion protein promoted histone acetylation of the target gene promoter and distal regulatory element and activated target gene expression more effectively and specifically (Figure 4 D,E). dCas9 p300-core (WT) has better inherent ligand adjustability than other CRISPRa transcriptional activation tools, which lays the ...
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Competitive dCas9 binding as a mechanism for transcriptional control
https://www.embopress.org/doi/full/10.15252/msb.202110512
WEBNov 8, 2021 · Abstract. Catalytically dead Cas9 (dCas9) is a programmable transcription factor that can be targeted to promoters through the design of small guide RNAs (sgRNAs), where it can function as an activator or repressor.
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dCas9-VPR-mediated transcriptional activation of functionally
https://www.nature.com/articles/s41596-021-00666-3
WEBFeb 7, 2022 · dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy | Nature Protocols. Protocol. Published: 07 February 2022. dCas9-VPR-mediated...
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dCas9 Tells Tales: Probing Gene Function and Transcription …
https://www.liebertpub.com/doi/full/10.1089/crispr.2023.0078
WEB3 days ago · Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing is evolving into an essential tool in the field of biological and medical research. Notably, the development of catalytically deactivated Cas9 (dCas9) enzyme has substantially broadened its traditional boundaries in gene editing or perturbation. The …
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CRISPR/dCas9 platforms in plants: strategies and applications beyond
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920162/
WEBSep 3, 2019 · Nuclease‐dead Cas9 ( dC as9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non‐functional. The applications of CRISPR / dC as9 have expanded and diversified in recent years.
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CRISPR/dCas9-Based Systems: Mechanisms and Applications in …
https://pubmed.ncbi.nlm.nih.gov/34685863/
WEBSep 29, 2021 · This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging.
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