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Frequently Asked Questions

What is potato dextrose agar?

Principle of Potato Dextrose Agar (PDA) Potato Dextrose Agar is composed of dehydrated Potato Infusion and Dextrose that encourage luxuriant fungal growth. Agar is added as the solidifying agent. Many standard procedures use a specified amount of sterile tartaric acid (10%) to lower the pH of this medium to 3.5 +/- 0.1, inhibiting bacterial growth.

Can Aspergillus grow in potato dextrose agar?

Aspergillus sp. growing in potato dextrose agar Potato dextrose agar (BAM Media M127) and potato dextrose broth are common microbiological growth media made from potato infusion, and dextrose. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria.

What is the pH of potato dextrose agar with chloramphenicol?

In addition, Potato Dextrose Agar with Chloramphenicol contains: Final pH of 5.6 +/- 0.2 at 25 degrees C. In addition, Potato Dextrose Agar with TA contains: Final pH of 3.5 +/- 0.3 at 25 degrees C. After sufficient incubation, isolated colonies should be visible in the streaked areas and confluent growth in areas of heavy inoculation.

How much dextrose is in a 200 GM potato infusion?

Note: 200 gm of potato infusion is equivalent to 4.0 gm of potato extract. Commercial PDA Powder (20 gm dextrose, 15 gm agar, and 4 gm potato starch) To prepare potato infusion, boil 200 g sliced, unpeeled potatoes in 1 liter distilled water for 30 min.


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