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Frequently Asked Questions

What is a coagulase test?

Coagulase Test. The coagulase test identifies whether an organism produces the exoenzyme coagulase, which causes the fibrin of blood plasma to clot.

What is the coagulase test for Staphylococcus aureus?

Coagulase test is used to differentiate Staphylococcus aureus (positive) from Coagulase Negative Staphylococcus (CONS). Coagulase is an enzyme produced by S. aureus that converts (soluble) fibrinogen in plasma to (insoluble) fibrin. Staphylococcus aureus produces two forms of coagulase, bound and free.

What happens if the tube coagulase test is false positive?

False-positive or false-negative results can occur if the plasma is not sterile. Report as Staphylococcus aureus if the tube coagulase test is positive and the organism is catalase-positive and a Gram-positive coccus in clusters.

Can Staphylococcus lugdunensis give positive slide coagulase test results?

Other species of Staphylococcus, namely, S. lugdunensis and S. schleiferi may give positive slide coagulase test results. Note: Colonies from a mannitol salt agar (MSA) culture are not suitable for coagulase testing. The organism must first be cultured on nutrient agar or blood agar. Slide Coagulase Test Procedure


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