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Text case is preserved, e.g. you can mark ATGs with lowercase. Instead of a sequence, you can paste a chromosome range, e.g. chr1:11,130,540-11,130,751
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Human Pluripotent Stem Cells (iPSC) Generation, Culture, and
Human HEK293 cell line (ATCC CRL1573 ... 38 It is important to use low passage HEK293T cells for the production of viruses. To make sure the cells are always in the fastest growth phase, never let the cells grow to 100 % confluence. 39 Prepare eight plates of HEK293T, ...
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ADAR1 RNA editing enzyme regulates R-loop formation and …
Mar 12, 2021 · We conducted immunoprecipitation experiments using HEK293T cells stably expressing FLAG-ADAR1p110-WT. ... (ATCC CCL-2), HEK293T human embryonic kidney (ATCC CRL-11268), HCT116 human colon ...
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Glycosylase base editors enable C-to-A and C-to-G base changes
Jul 20, 2020 · HEK293T cells from the American Type Culture Collection (ATCC) were cultured in DMEM supplemented with 10% (vol/vol) FBS at 37 °C under 5% CO 2. Cells were seeded in 24-well plates (Corning).
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Deubiquitinase USP8 increases ID1 stability and promotes …
All the cells were grown in RPMI/1640 medium (HyClone, USA) supplemented with 10% fetal bovine serum (HyClone, USA). HEK293T cell line was purchased from American Type Culture Collection (ATCC) and cultured in DMEM (HyClone, USA) plus with 10% fetal bovine serum. 2.2. Plasmids and antibodies
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Tandem CAR-T cells targeting CD70 and B7-H3 exhibit potent …
Jun 18, 2020 · The NCI-H460, A375, MDA-MB-435, 786-O, Fadu and K562 tumor cell lines were purchased from the American Type Culture Collection (ATCC). A375 cell line with B7-H3 gene knocked out was produced using a CRISPR-Cas9 system. ... Aliquots of lentivirus were stored at -80 °C and lentiviral particle titers were measured by transduction of HEK293T cells ...
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Ovarian cancer immunogenicity is governed by a narrow subset of
May 09, 2022 · K562 cells (ATCC Cat# CCL-243, RRID:CVCL_0004), HEK293T cells (ATCC Cat# CRL-3216, RRID:CVCL_0063) and Phoenix-AMPHO cells (ATCC Cat# SD-3443, RRID:CVCL_H716) were purchased from ATCC (Manassas, VA). Jurkat76 cells were provided by Mirjam H. Heemskerk (Department of Hematology, University of Leiden, Leiden, …
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Immune recall improves antibody durability and breadth to SARS …
May 12, 2022 · HEK293T cell line (ATCC) was maintained in DMEM containing 10% FBS, 100 U/mL penicillin and 100 U/mL streptomycin (Gibco). HEK293T stable cell line overexpressing human ACE2 and TMRPSS2 (ACE2/TMPRSS2-expressing HEK293T) was kindly provided by Dr. Marc Johnson (University of Missouri School of Medicine). ... Cells were harvested 2 days post ...
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