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Transfection of 293F Mammalian Cells using PEI - UGA
HEK293S GnTI- cell line (ATCC cat. no. CRL-3022) Ex-Cell® 293 Serum-Free Medium (Sigma cat. no. 14571C) ESF serum free medium (Expression Systems cat. no. 98-001) Suspension adapted HEK293S GnTI- cells grow well in Ex-Cell 293 media or in a 50:50 mix of Ex-Cell and Freestyle™ 293 medium, but do not grow well on Freestyle™ 293 medium alone.
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Low-dose self-amplifying mRNA COVID-19 vaccine drives strong …
Jun 07, 2022 · The virus was amplified, harvested, and re-infected into 30 mL of HEK293F cells for 48–72 h. Cells and media were harvested and used to infect 400 mL of HEK293F cells.
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Myosin 1D and the branched actin network control the …
Apr 27, 2022 · NRK cells were obtained from the American Type Culture Collection (ATCC). Cells ... Protein expression and purification. pCAG-strep-Myosin1D vector was transfected into HEK293F cells using a ...
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Facile discovery of surrogate cytokine agonists: Cell
Mar 23, 2022 · ATCC: Cat#CRL-1711: Insect: Trichoplusia ni (T. ni) Expression Systems: Cat#94-002F: Human: YT-1 (CD25+) Kuziel et al., 1993. ... and IFNAR2 ECD (a.a. 27-243) were expressed as Fc fusions in HEK293F cells and purified by protein A affinity chromatography. Purified receptor ECDs were mixed with Freund's adjuvant, then individually injected into ...
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Broad neutralization of SARS-CoV-2 variants by an inhalable …
Vero E6 cells and 293 T cells were obtained from the American Type Culture Collection (ATCC), Huh-7 cells were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). ... The expression plasmid was transiently transfected into suspension HEK293F by polyethylenimine. After 72 hours, the supernatants were harvested and ...
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