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293T/17 [HEK 293T/17] | ATCC
https://www.atcc.org/products/crl-11268
Sep 14, 1999 · 293T/17 [HEK 293T/17] is a cell line exhibiting epithelial morphology that was isolated from human embryo kidney tissue. The cell line is a derivative of the 293T (293tsA1609neo) cell line, a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the …
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HEK293 Cell Culture and Transfection | HEK293 Cell Line
https://www.hek293.com/cell-subculture-protocol/
HEK293 are rounded cells that grow in suspension in cell culture, although initially they were an adherent cell line. HEK-293 cells should be grown in a complete SFMII growth medium supplemented with 4 mM L-glutamine. Flasks should be incubated at 37°C in 5% CO2 and HEK293 cell doubling time is approximately 34 hours. ... Purchase HEK293 (ATCC ...
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Transfection of 293F Mammalian Cells using PEI - UGA
http://glycoenzymes.ccrc.uga.edu/MammalianExpression/MammalianTransfectionPEI.pdf
Cells are grown in suspension on a platform shaker in a humidified 37°C CO 2 incubator with rotation at ~150 rpm. Maintain cultures for 5-7 passages prior to performing transfections to ensure stable growth patterns. When using suspension-adapted HEK293 cells (Freestyle™ 293-F cells, Life Technologies, Cat. No. R790-07) grow the
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A small and highly sensitive red/far-red optogenetic switch for
https://www.nature.com/articles/s41587-021-01036-w
Oct 04, 2021 · HEK293 cells (CRL-1573, ATCC), human cervical adenocarcinoma cells (HeLa; CCL-2, ATCC), HEK293-derived Hana3A cells engineered for constitutive expression of …
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Jurkat Cell Line - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/immunology-and-microbiology/jurkat-cell-line
Monty Montano, in Translational Biology in Medicine, 2014. 2.2.3 Infection and immunity. The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14].The Jurkat cell line has most often been used as a prototypical T cell line to study multiple events in T cell biology, including a) T cell …
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Engineering circular RNA for potent and stable translation in
https://www.nature.com/articles/s41467-018-05096-6/
Jul 06, 2018 · HEK293, HEK293-GFP, HeLa, and A549 cells (ATCC) were cultured at 37 °C and 5% CO 2 in Dulbecco’s Modified Eagle’s Medium (4500 mg/L glucose) supplemented with 10% heat-inactivated fetal ...
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CRISPOR
http://crispor.tefor.net/crispor.py
Text case is preserved, e.g. you can mark ATGs with lowercase. Instead of a sequence, you can paste a chromosome range, e.g. chr1:11,130,540-11,130,751
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团队作案HEK293，史上最强搬砖细胞 - 简书
https://www.jianshu.com/p/cf686abe846e
Apr 21, 2020 · 与HEK293-EBNA相比，其产生重组蛋白的能力增强。 5. 293 F. 293-F是293细胞系的一个快速增长的衍生细胞系，GIBCO说可以用于无菌悬浮培养。 5.1 293FT cells. 293FT细胞由293F细胞系中插入SV40 large T antigen，其特点是增殖速度快，易转染，主要用于用于慢病毒的 …
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Non-cleavable hinge enhances avidity and expansion of CAR-T cells …
https://www.cell.com/cancer-cell/fulltext/S1535-6108(22)00157-X
Apr 21, 2022 · Fresh PDX cells (5 × 10 6) were injected on day −4 followed by 2 × 10 6 CAR-T cells either native or CD8H&TM on day 0. Serial blood monitoring by flow cytometry revealed steady tumor growth in the untreated and native CAR-T cell-treated mice with low-level delayed CAR-T cell expansion in the latter.
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