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HEK293 Cell Culture and Transfection | HEK293 Cell Line
HEK293 are rounded cells that grow in suspension in cell culture, although initially they were an adherent cell line. HEK-293 cells should be grown in a complete SFMII growth medium supplemented with 4 mM L-glutamine. Flasks should be incubated at 37°C in 5% CO2 and HEK293 cell doubling time is approximately 34 hours. Subculture Protocol for ...
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293T/17 [HEK 293T/17] | ATCC
Sep 14, 1999 · The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, …
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Jurkat Cell Line - an overview | ScienceDirect Topics
Monty Montano, in Translational Biology in Medicine, 2014. 2.2.3 Infection and immunity. The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia .The Jurkat cell line has most often been used as a prototypical T cell line to study multiple events in T cell biology, including a) T cell …
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The SH-SY5Y cell line in Parkinson’s disease research: a …
Jan 24, 2017 · The most-reported source for access to the SH-SY5Y cell line is the American Type Culture Collection (ATCC, CRL-2266, deposited by JL Biedler). ... such as HEK293, HeLa or glial cells. Primary cell cultures are physiologically more relevant than immortalized cell lines, but they are difficult to maintain and, ... The Neuro-2a cell line (ATCC ...
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Transfection of 293F Mammalian Cells using PEI - UGA
HEK293S GnTI- cell line (ATCC cat. no. CRL-3022) Ex-Cell® 293 Serum-Free Medium (Sigma cat. no. 14571C) ESF serum free medium (Expression Systems cat. no. 98-001) Suspension adapted HEK293S GnTI- cells grow well in Ex-Cell 293 media or in a 50:50 mix of Ex-Cell and Freestyle™ 293 medium, but do not grow well on Freestyle™ 293 medium alone ...
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A small and highly sensitive red/far-red optogenetic switch for
Oct 04, 2021 · HEK293 cells (CRL-1573, ATCC), human cervical adenocarcinoma cells ... a HEK REDMAP-SEAP-P2A-mINS cell line for red light-inducible SEAP and insulin production was constructed by cotransfecting ...
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Taxonomy browser (Homo sapiens)
THE NCBI Taxonomy database allows browsing of the taxonomy tree, which contains a classification of organisms.
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Text case is preserved, e.g. you can mark ATGs with lowercase. Instead of a sequence, you can paste a chromosome range, e.g. chr1:11,130,540-11,130,751
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团队作案HEK293，史上最强搬砖细胞 - 简书
Apr 21, 2020 · HEK293 or 293 cells 在1973年，科学家从一个父母谱系不祥的人类流产胚胎的肾脏中分离出最原始的293细胞（human embryonic kidney (HEK) 293 cell line），该细胞被转入修改后的5型腺病毒DNA片段，一开始转化非常困难，一直到几个月后，科学家得到一个稳定的可以快速生长的293 ...
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A non-canonical cGAS–STING–PERK pathway facilitates the …
May 02, 2022 · HEK293, HeLa, HCT116 and DLD1 cells were obtained from ATCC. ... No cell lines used in this study were found in the commonly misidentified cell lines maintained by the International Cell Line ...
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