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ATAC-seq - Wikipedia
https://en.wikipedia.org/wiki/ATAC-seq
WEBATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility. In 2013, the technique was first described as an alternative advanced method for MNase-seq, FAIRE-Seq and DNase-Seq.
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Chromatin accessibility profiling by ATAC-seq | Nature Protocols
https://www.nature.com/articles/s41596-022-00692-9
WEBOct 26, 2020 · The assay for transposase-accessible chromatin using sequencing (ATAC-seq) provides a simple and scalable way to detect the unique chromatin landscape associated with a cell type and how it...
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Complete Guide to Understanding and Using ATAC-Seq - Active …
https://www.activemotif.com/blog-atac-seq
WEBFeb 9, 2021 · This article discusses what ATAC-Seq is, it’s history, how it works, and some discoveries enabled by ATAC-Seq. What is ATAC-Seq? ATAC-Seq stands for Assay for Transposase-Accessible Chromatin with high-throughput sequencing. The ATAC-Seq method relies on next-generation sequencing (NGS) library construction using the …
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ATAC-Seq for Chromatin Accessibility Analysis | Illumina
https://www.illumina.com/techniques/multiomics/epigenetics/atac-seq-chromatin-accessibility.html
WEBThe assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome. By sequencing regions of open chromatin, ATAC-Seq can help you uncover how chromatin packaging and other factors affect gene expression.
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From reads to insight: a hitchhiker’s guide to ATAC-seq data …
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-1929-3
WEBFeb 3, 2020 · Briefly, ATAC-seq incorporates a genetically engineered hyperactive Tn5 transposase that simultaneously cuts open chromatin leaving a 9-bp staggered nick and ligates high-throughput sequencing adapters to these regions. During this process, the nick is repaired, leaving a 9-bp duplication [ 30, 31 ].
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Chromatin accessibility profiling by ATAC-seq - Nature
https://www.nature.com/articles/s41596-022-00692-9.pdf
WEBThe assay for transposase-accessible chromatin using sequencing (ATAC-seq) provides a simple and scalable way to detect the unique chromatin landscape associated with a cell type and how it may be ...
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ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide
https://pubmed.ncbi.nlm.nih.gov/25559105/
WEBJan 5, 2015 · This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin.
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Analytical Approaches for ATAC-seq Data Analysis - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191135/
WEBA typical ATAC-seq analysis can be divided into two major components: (1) general processing of raw sequencing reads, which produces intermediate outputs like annotated peak calls; and (2) detailed downstream analysis, which is more specific to a particular biological question ( Fig. 2 ).
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An ATAC-seq atlas of chromatin accessibility in mouse tissues
https://www.nature.com/articles/s41597-019-0071-0
WEBMay 20, 2019 · The Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) is a fundamental epigenomics approach and has been widely used in profiling the chromatin accessibility dynamics in...
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Hands-on: ATAC-Seq data analysis / Epigenetics - Galaxy …
https://training.galaxyproject.org/training-material/topics/epigenetics/tutorials/atac-seq/tutorial.html
WEBSep 2, 2019 · In this training you have learned the general principles of ATAC-Seq data analysis. ATAC-Seq is a method to investigate the chromatin accessibility and the genome is treated with a transposase (enzyme) called Tn5. It marks open chromatin regions by cutting and inserting adapters for sequencing.
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