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Addgene: CRISPR Guide
https://www.addgene.org/guides/crispr/
WebThis guide will provide a basic understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR in your own research. Generating a Knockout Using CRISPR
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Addgene: CRISPR Plasmids and Resources
https://www.addgene.org/crispr/
WebCRISPR Plasmids and Resources. Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.
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Addgene: CRISPR Pooled gRNA Libraries
https://www.addgene.org/crispr/libraries/
WebCRISPR Pooled Libraries. CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells are ...
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CRISPR History and Development for Genome Engineering - Addgene
https://www.addgene.org/crispr/history/
WebCRISPR: An Adaptive Immune System. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007.
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Addgene: CRISPR Plasmids - Tagging
https://www.addgene.org/crispr/tagging/
WebThe CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for improved efficiency and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed for using them, are described below.
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Addgene: CRISPR References and Information
https://www.addgene.org/crispr/reference/
WebCRISPResso is a robust and easy-to-use computational pipeline that enables accurate quantification and visualization of CRISPR-Cas9 outcomes, as well as comprehensive evaluation of their effect on the coding sequence, functional noncoding elements and …
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Addgene: CRISPR Plasmids - Activate Gene Expression
https://www.addgene.org/crispr/activate/
WebBrowse, sort, or search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.
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Addgene: CRISPR Plasmids - Mammalian Expression
https://www.addgene.org/crispr/mammalian/
WebThe following CRISPR plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence.
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CRISPR Topic Overview Page - Addgene
https://info.addgene.org/crispr-topic-page
WebThe Class 2 Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) system is an adaptive immune system in bacteria. It's been modified for genome engineering and used for many other applications in biology. From base editing, to prime editing, to RNA editing, there's a lot of ways that CRISPR can be used. Learn more!
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Twenty Years of Addgene Sharing: CRISPR
https://blog.addgene.org/twenty-years-of-addgene-crispr
Web5 days ago · Addgene’s CRISPR collection contained 197 plasmids in 2013. A decade later, it had grown to 17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost 300,000 plasmids with researchers all over the world.
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