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A-375 [A375] - CRL-1619 | ATCC
A-375 [A375] is a cell line exhibiting epithelial morphology that was isolated from the skin of a 54-year-old, female patient with malignant melanoma. This cell line is the ideal control for NRAS mutant-A375 isogenic cell line (ATCC CRL-1619IG-2) and can be used in toxicology and immuno-oncology research.
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ADAR1 masks the cancer immunotherapeutic promise of ZBP1
May 25, 2022 · a, HepG2, MCF7, A549, HT-29, HeLa, SK-MEL-2 or A375 human cell lines were treated with CBL0137 (5 µM) for 12 h and stained for Z-DNA. b , Quantification of fluorescence intensity of Z-DNA signal ...
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Luciferase-labeled Cells | ATCC
ATCC luciferase-reporter cell lines: Establish in vivo tumor models ; ... This cell line is essential for in vivo bioluminescence imaging of xenograft animal models to study human lung cancer and monitor cancer drugs. Get the Cells. Human A375-Luc2 Ideal for in vivo bioluminescence imaging to study human melanoma and monitor activity of ...
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ATCC: The Global Bioresource Center
ATCC has created the first reporter cell lines via CRISPR/Cas9 genome editing for real-time monitoring of EMT. hTERT-immortalized NTAP Schwann Cells. ... CRISPR-edited MEK1 Q56P Mutant A375 cell line. NRAS Isogenic Cells. CRISPR-edited …
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Systematic discovery of mutation-directed neo-protein-protein
May 26, 2022 · To systematically discover Df-PPIs in live cells, a differential screening platform, qHT-dS, was established (Figures 1B and S1).It is enabled by using BRET n-based ultra-high-throughput PPI screening technology in a 1536 well plate format (Mo and Fu, 2016; Mo et al., 2016) (Figure S1A). Briefly, the WT and MUT proteins, genetically tagged with Nanoluc …
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Systematic discovery of mutation-directed neo-protein-protein ... - Cell
May 04, 2022 · Five neoPPIs were further confirmed with an endogenous flag-V600E co-IP study in a colon cancer cell line (Figure 4Ei). (iii) co-IP quality antibodies were utilized to demonstrate endogenous BRAF V600E complexes with VHL, BCL2L1, and KEAP1 in a native state of patient-derived cancer cells harboring BRAF V600E ( Figure 4 Eii).
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CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as
Mar 25, 2022 · MAGeCK analysis for genes that generally affect A375 cell fitness in the ... (NY-ESO-1 +, HLA-A2 −; ATCC CRL-5896), H1299 non-small cell lung ... The cancer cell line encyclopedia enables ...
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Reprogramming human T cell function and specificity with non …
Jul 11, 2018 · NSG mice (8–12 weeks old) were seeded with 1 × 10 6 A375 cells (human melanoma cell line; NY-ESO-1 antigen + and HLA-A*0201 +) subcutaneously in a shaved flank. Primary human T cells edited to ...
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Optimized libraries for CRISPR-Cas9 genetic screens with ... - Nature
Dec 21, 2018 · Cell culture. A375, HT29, and MelJuSo cells were obtained from the Cancer Cell Line Encyclopedia. ... HEK293Ts were obtained from ATCC (CRL-3216). All cell lines were routinely tested for ...
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Targeting the transcriptional activity of STAT3 by a novel fusion
Jul 10, 2022 · Cell lines and reagents. Cell lines A375 and HeLa were purchased from (American Type Culture Collection, ATCC) and grown at 37 °C in the presence of 5% CO 2 in Dulbecco’s modified Eagle’s medium (DMEM, Biological Industries 01-052-1A) with high glucose and supplemented with 10% fetal bovine serum (FBS, Biological Industries 04-007-1A), 2 ...
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