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293T - CRL-3216 | ATCC
https://www.atcc.org/products/crl-3216
WEBIncubate the culture at 37°C in a suitable incubator. A 5% CO 2 in air atmosphere is recommended if using the medium described on this product. Subculturing procedure Volumes used in this protocol are for 75 cm 2 …
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Evolution from adherent to suspension: systems biology of …
https://www.nature.com/articles/s41598-020-76137-8
WEBNov 4, 2020 · The adherent cell lines HEK293 (ATCC-CRL-1573), HEK293T (ATCC-CRL-3216) and 293E (ATCC-CRL-10852) were obtained from ATCC and propagated in … Author: Magdalena Malm, Rasool Saghaleyni, Magnus Lundqvist, Marco Giudici, Veronique Chotteau, Ray Field, P... Publish Year: 2020
Author: Magdalena Malm, Rasool Saghaleyni, Magnus Lundqvist, Marco Giudici, Veronique Chotteau, Ray Field, P...
Publish Year: 2020
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Growth and Maintenance of the 293FT Cell Line | Thermo Fisher
https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/transfection-protocol/growth-and-maintenance-of-the-293ft-cell-line.html
WEBThe table below lists the recommended complete medium, freezing medium, and antibiotic concentration required to maintain and culture the 293FT Cell Line. Note: Fetal bovine …
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HEK 293T Tissue Culture Protocols - Nutrition, Dietetics, & Food …
https://ndfs.byu.edu/tessem-lab/hek-293t-tissue-culture-protocols
WEBChill freezing media for 3-5 minutes. Add 7 mL media to trypsinized solution. Mix. Repeatedly suck solution into pipet, and then spray back into the flask, thus breaking up …
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Human ACE2 293T Cell Line Protocol-At-A-Glance - Takara Bio
https://www.takarabio.com/documents/User%20Manual/Human%20ACE2%20293T%20Cell%20Line%20Protocol-at-a-Glance_021121.pdf
WEBThe Human ACE2 293T Cell Line (Cat. No. 631289) should be cultured immediately upon receipt, or as soon as possible thereafter. Significant delays in culturing may result in … File Size: 175KB Page Count: 2
File Size: 175KB
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Lenti-X™ 293T Cell Line Protocol-At-A-Glance - Takara Bio
https://www.takarabio.com/documents/User%20Manual/Lenti/Lenti-X%20293T%20Cell%20Line%20Protocol-at-a-Glance_050514.pdf
WEBThe Lenti-X 293T Cell Line (Cat. No. 632180) should be cultured immediately upon receipt, or as soon as possible thereafter. If culturing is significantly delayed after receipt, … File Size: 152KB Page Count: 2
File Size: 152KB
Page Count: 2
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Protocol for cell preparation and gene delivery in HEK293T and …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313743/
WEBSep 9, 2021 · Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene …
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HEK293 Cells: Background, Applications, Protocols, …
https://www.synthego.com/hek293
WEBThe HEK293 cell line consists of immortalized human embryonic kidney cells is one of the most widely used cell lines in research. Since its establishment in the 1970s, this robust and fast-growing cell line and its …
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Growth and Maintenance of the 293FT Cell Line - WPMU DEV
https://cpb-us-e1.wpmucdn.com/sites.psu.edu/dist/c/147165/files/2022/03/293ft_cells_man.pdf
WEBUse the following procedure to thaw 293FT cells to initiate cell culture. Thaw cells in prewarmed, complete medium . without. Geneticin ®. 1. Remove the vial of frozen cells from liquid nitrogen and thaw quickly in a 37°C water bath. 2. Just before the cells are completely thawed, decontaminate the outside of the
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HEK293T Cell Line - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/immunology-and-microbiology/hek293t-cell-line
WEBHEK293T Cell Line - an overview | ScienceDirect Topics. Ion Channels: Channel Production and Optical Methods. Kate Dunning, Thomas Grutter, in Methods in …
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